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| Product Name | CD195 [6G11D1] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | AM4 7 antibody C C chemokine receptor type 5 antibody C C CKR 5 antibody C-C chemokine receptor type 5 antibody C-C CKR-5 antibody C-C motif chemokine receptor 5 A159A antibody CC Chemokine Receptor 5 antibody CC Chemokine Receptor Type 5 antibody CC CKR 5 antibody CC-CKR-5 antibody CCCKR 5 antibody CCCKR5 antibody CCR 5 antibody CCR-5 antibody CCR5 antibody CCR5 chemokine (C C motif) receptor 5 antibody CCR5_HUMAN antibody CD 195 antibody CD195 antibody CD195 Antigen antibody Chemokine C C motif receptor 5 antibody Chemokine receptor CCR5 antibody CHEMR13 antibody CKR 5 antibody CKR5 antibody CMKBR 5 antibody CMKBR5 antibody FLJ78003 antibody HIV 1 Fusion Coreceptor antibody HIV-1 fusion coreceptor antibody HIV1 fusion coreceptor antibody IDDM22 antibody MIP-1 alpha receptor antibody |
| Product description | This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. |
| Immunogen | Purified recombinant fragment of human CD195 (AA: extra mix) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/mL |
| Alternative Names | AM4 7 antibody C C chemokine receptor type 5 antibody C C CKR 5 antibody C-C chemokine receptor type 5 antibody C-C CKR-5 antibody C-C motif chemokine receptor 5 A159A antibody CC Chemokine Receptor 5 antibody CC Chemokine Receptor Type 5 antibody CC CKR 5 antibody CC-CKR-5 antibody CCCKR 5 antibody CCCKR5 antibody CCR 5 antibody CCR-5 antibody CCR5 antibody CCR5 chemokine (C C motif) receptor 5 antibody CCR5_HUMAN antibody CD 195 antibody CD195 antibody CD195 Antigen antibody Chemokine C C motif receptor 5 antibody Chemokine receptor CCR5 antibody CHEMR13 antibody CKR 5 antibody CKR5 antibody CMKBR 5 antibody CMKBR5 antibody FLJ78003 antibody HIV 1 Fusion Coreceptor antibody HIV-1 fusion coreceptor antibody HIV1 fusion coreceptor antibody IDDM22 antibody MIP-1 alpha receptor antibody |
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| Molecular Weight(MW) | 40.5kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P51681 |
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Application
Western blot analysis of CD195 against human CD195 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD195 against HEK293 (1) and CD195 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD195 against MOLT4 (1), L-02 (2), SPA-C-1 (3), A549 (4), and C6 (5) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-CD195 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-50, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using anti-CD195 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-50, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of CD195 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-50, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | MOLT4, L-02, SPA-C-1, A549, and C6 cell lysate, K562 cells, cervical cancer tissues, bladder cancer tissues |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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