Signal regulatory protein alpha type 1 antibody Bit antibody Brain Ig like molecule with tyrosine based activation motifs antibody Brain Ig-like molecule with tyrosine-based activation motifs antibody Brain immunoglobulin like molecule with tyrosine based activation motifs antibody CD172 antigen like family member A antibody CD172 antigen-like family member A antibody CD172a antibody CD172a antigen antibody Inhibitory receptor SHPS-1 antibody Macrophage fusion receptor antibody MFR antibody MYD 1 antibody Myd 1 antigen antibody MyD-1 antigen antibody p84 antibody Protein tyrosine phosphatase non receptor type substrate 1 antibody PTPNS1 antibody SHP substrate 1 antibody SHPS-1 antibody SHPS1 antibody SHPS1_HUMAN antibody Signal regulatory protein alpha 2 antibody Signal regulatory protein alpha 3 antibody Signal regulatory protein alpha antibody Signal regulatory protein alpha type 2 antibody Signal-regulatory protein alpha-1 antibody Signal-regulatory protein alpha-2 antibody Signal-regulatory protein alpha-3 antibody SIRP antibody Sirp-alpha-1 antibody Sirp-alpha-2 antibody Sirp-alpha-3 antibody SIRPA antibody SIRPalpha antibody SIRPalpha1 antibody SIRPalpha2 antibody SIRPalpha3 antibody Tyrosine phosphatase SHP substrate 1 antibody Tyrosine protein phosphatase non receptor type substrate 1 antibody Tyrosine-protein phosphatase non-receptor type substrate 1 antibody
Product description
The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.
Immunogen
Purified recombinant fragment of human CD172A (AA: extra 235-373) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBFC
WB:1:500-1:2,000 FC:1:100-1:200
Species Reactivity
Human
Concentration
1mg/mL
Target Information
Alternative Names
Signal regulatory protein alpha type 1 antibody Bit antibody Brain Ig like molecule with tyrosine based activation motifs antibody Brain Ig-like molecule with tyrosine-based activation motifs antibody Brain immunoglobulin like molecule with tyrosine based activation motifs antibody CD172 antigen like family member A antibody CD172 antigen-like family member A antibody CD172a antibody CD172a antigen antibody Inhibitory receptor SHPS-1 antibody Macrophage fusion receptor antibody MFR antibody MYD 1 antibody Myd 1 antigen antibody MyD-1 antigen antibody p84 antibody Protein tyrosine phosphatase non receptor type substrate 1 antibody PTPNS1 antibody SHP substrate 1 antibody SHPS-1 antibody SHPS1 antibody SHPS1_HUMAN antibody Signal regulatory protein alpha 2 antibody Signal regulatory protein alpha 3 antibody Signal regulatory protein alpha antibody Signal regulatory protein alpha type 2 antibody Signal-regulatory protein alpha-1 antibody Signal-regulatory protein alpha-2 antibody Signal-regulatory protein alpha-3 antibody SIRP antibody Sirp-alpha-1 antibody Sirp-alpha-2 antibody Sirp-alpha-3 antibody SIRPA antibody SIRPalpha antibody SIRPalpha1 antibody SIRPalpha2 antibody SIRPalpha3 antibody Tyrosine phosphatase SHP substrate 1 antibody Tyrosine protein phosphatase non receptor type substrate 1 antibody Tyrosine-protein phosphatase non-receptor type substrate 1 antibody
Western blot analysis of CD172A against human CD172A (AA: extra 235-373) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD172A against HEK293 (1) and CD172A (AA: extra 235-373)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD172A was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-64, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Positive Control
Ramos cells
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
