ADH water channel antibody AQP 2 antibody AQP CD antibody AQP-2 antibody AQP-CD antibody AQP2 antibody AQP2_HUMAN antibody AQPCD antibody Aquaporin 2 collecting duct antibody Aquaporin CD antibody Aquaporin-2 antibody Aquaporin-CD antibody Aquaporin2 antibody Aquaporine 2 antibody Collecting duct water channel protein antibody MGC34501 antibody Water channel aquaporin 2 antibody Water channel protein for renal collecting duct antibody WCH CD antibody WCH-CD antibody WCHCD antibody
Product description
AQP2 is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. It is the only aquaporin regulated by vasopressin.[5] The basic job of aquaporin 2 is to reabsorb water from the urine while its being removed from the blood by the kidney. Aquaporin 2 is in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed, vasopressin binds to the cell surface vasopressin receptor thereby activating a signaling pathway that causes the aquaporin 2 containing vesicles to fuse with the plasma membrane, so the aquaporin 2 can be used by the cell. Mutations in this channel are associated with nephrogenic diabetes insipidus, which can be autosomal dominant or recessive. Mutations in the vasopressin receptor cause a similar X-linked phenotype. Lithium, which is often used to treat bipolar disorder, can cause acquired diabetes insipidus (characterized by the excretion of large volumes of dilute urine) by decreasing the expression of the AQP2 gene. The expression of the AQP2 gene is increased during conditions associated with water retention such as pregnancy and congestive heart failure.
Immunogen
Purified recombinant fragment of human AQP2 (AA: 149-271) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WB
WB:1:500-1:2,000
Species Reactivity
Human
Concentration
1mg/mL
Target Information
Alternative Names
ADH water channel antibody AQP 2 antibody AQP CD antibody AQP-2 antibody AQP-CD antibody AQP2 antibody AQP2_HUMAN antibody AQPCD antibody Aquaporin 2 collecting duct antibody Aquaporin CD antibody Aquaporin-2 antibody Aquaporin-CD antibody Aquaporin2 antibody Aquaporine 2 antibody Collecting duct water channel protein antibody MGC34501 antibody Water channel aquaporin 2 antibody Water channel protein for renal collecting duct antibody WCH CD antibody WCH-CD antibody WCHCD antibody
Molecular Weight(MW)
28.8kDa
Cellular Localization
Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
Western blot analysis of AQP2 against human AQP2 (AA: 149-271) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of AQP2 against HEK293 (1) and AQP2 (AA: 149-271)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of AQP2 against K562 (1), HeLa (2), HCT116 (3), and SW480 (4) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Positive Control
K562, HeLa, HCT116, and SW480 cell
Application Notes
WB:1:500-1:2,000
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
