Acidic and basic fibroblast growth factors (FGFs) are members of a family of multifunctional polypeptide growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Like other growth factors, FGFs act by binding and activating specific cell surface receptors. These include the Flg receptor (FGFR-1), the Bek receptor (FGFR-2), FGFR-3, FGFR-4, FGFR-5 and FGFR-6. These receptors usually contain an extracellular ligand-binding region containing three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The gene encoding human Flg maps to chromosome 8p12 and is alternatively spliced to produce several isoforms. Mutations in Flg are associated with Pfeiffer syndrome (a skeletal disorder characterized by craniosynostosis with deviation and enlargement of the thumbs and great toes), brachymesophalangy with phalangeal ankylosis and a varying degree of soft tissue syndactyly. The Flg gene is also involved in chromosomal translocations with ZNF198, CEP110 and FOP, which may lead to stem cell leukemia lymphoma (SCLL).
Fig1: Western blot analysis of FGFR1 on 293 cells lysates using anti-FGFR1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining FGFR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining FGFR1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Positive Control
293, 293T, Hela.
Application Notes
WB:1:1,000 ICC:1:50-1:100
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
