| 存储条件 |
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| Product Name | IRAK4 [B9-H2] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-1 receptor-associated kinase 4 antibody Interleukin 1 receptor associated kinase 4 mutant form 1 antibody Interleukin-1 receptor-associated kinase 4 antibody Interleukin1 receptor associated kinase 4 antibody IPD1 antibody IRAK 4 antibody IRAK-4 antibody IRAK4 antibody IRAK4 mutated form 1 antibody IRAK4_HUMAN antibody LOC 51135 antibody NY REN 64 antibody NY REN 64 antigen antibody NY-REN-64 antibody REN64 antibody Renal carcinoma antigen NY-REN-64 antibody |
| Product description | Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs. IRAK family members include two active kinases, IRAK-1 and IRAK-4, and two inactive kinases, IRAK-2 and IRAK-M. Binding of IL-1 to its cognate receptor results in the activation of the NFkB signaling pathway and MAP kinase pathways. IRAK-4 appears to act upstream of other IRAKs and phosphorylates IRAK-1 on threonine 387. It is highly expressed in liver and kidney tissues, but also displays a wide, low level of expression in other tissues. IRAK-4 is an essential component of innate immunity. Deficiency of IRAK-4 leads to recurrent bacterial infections and profound hyporesponsiveness to LPS and IL-1. Therefore, IRAK-4 may be a potential target for therapeutic drug design. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | HumanMouseMonkey |
| Concentration | 2mg/ml |
| Alternative Names | IL-1 receptor-associated kinase 4 antibody Interleukin 1 receptor associated kinase 4 mutant form 1 antibody Interleukin-1 receptor-associated kinase 4 antibody Interleukin1 receptor associated kinase 4 antibody IPD1 antibody IRAK 4 antibody IRAK-4 antibody IRAK4 antibody IRAK4 mutated form 1 antibody IRAK4_HUMAN antibody LOC 51135 antibody NY REN 64 antibody NY REN 64 antigen antibody NY-REN-64 antibody REN64 antibody Renal carcinoma antigen NY-REN-64 antibody |
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| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of IRAK4 on human IRAK4 recombinant protein using anti-IRAK4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of IRAK4 on different cell lysates using anti-IRAK4 antibody at 1/1,000 dilution. Positive control: Lane 1: THP-1 Lane 2: Hela Lane 3: K562 Lane 4: MCF-7 Lane 5: RAW264.7 Lane 6: Jurkat Lane 7: Cos7
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-IRAK4 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue using anti-IRAK4 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with IRAK4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human IRAK4 recombinant protein, THP-1, Hela, K562, MCF-7, RAW264.7, Jurkat, Cos7, human lung cancer tissue, human kidney cancer tissue. |
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| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
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