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| Product Name | SIRT2 [SN70-04] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ35621 antibody FLJ37491 antibody NAD dependent deacetylase sirtuin 2 antibody NAD-dependent deacetylase sirtuin-2 antibody NAD-dependent protein deacetylase sirtuin-2 antibody Regulatory protein SIR2 homolog 2 antibody Silencing information regulator 2 like antibody Silent information regulator 2 antibody SIR2 antibody SIR2 like protein 2 antibody Sir2 related protein type 2 antibody SIR2, S. cerevisiae, homolog-loke 2 antibody SIR2-like protein 2 antibody SIR2L antibody SIR2L2 antibody SIRT2 antibody SIRT2_HUMAN antibody Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody Sirtuin 2 antibody Sirtuin type 2 antibody |
| Product description | The silent information regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in a NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT proteins may function via mono-ADP-ribosylation of proteins. SIRT2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIPFC |
| ICC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FLJ35621 antibody FLJ37491 antibody NAD dependent deacetylase sirtuin 2 antibody NAD-dependent deacetylase sirtuin-2 antibody NAD-dependent protein deacetylase sirtuin-2 antibody Regulatory protein SIR2 homolog 2 antibody Silencing information regulator 2 like antibody Silent information regulator 2 antibody SIR2 antibody SIR2 like protein 2 antibody Sir2 related protein type 2 antibody SIR2 S. cerevisiae homolog-loke 2 antibody SIR2-like protein 2 antibody SIR2L antibody SIR2L2 antibody SIRT2 antibody SIRT2_HUMAN antibody Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody Sirtuin 2 antibody Sirtuin type 2 antibody |
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| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Nucleus, Cytoplasm, Midbody, Chromosome, Cell projection, Myelin membrane. |
Application
Fig1: ICC staining SIRT2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining SIRT2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of Hela cells with SIRT2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | 293, PC-12, Hela. |
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| Application Notes | ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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