| 存储条件 |
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| Product Name | DDX3X |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP dependent RNA helicase DDX3X antibody ATP-dependent RNA helicase DDX3X antibody CAP Rf antibody DBX antibody DDX14 antibody DDX3X antibody DDX3X_HUMAN antibody DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody DEAD box antibody DEAD box protein 3 antibody DEAD box protein 3 X-chromosomal antibody DEAD box X isoform antibody DEAD box, X isoform antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody DEAD/H box 3 antibody DEAD/H box 3, X-linked antibody Helicase like protein 2 antibody Helicase-like protein 2 antibody HLP2 antibody X isoform antibody X-chromosomal antibody |
| Product description | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3 (DEAD box polypeptide 3) is involved in RNA metabolism. Two DDX3 paralogs are found in humans; DDX3X is encoded by a gene found on the X chromosome while DDX3Y is encoded by a gene on the Y chromosome. DDX3Y is exclusively expressed in testis and is required for normal spermatogenesis. DDX3X is ubiquitously expressed and predominantly localizes to the nuclear speckles, participating in RNA splicing, transcription, translation initiation, mRNA transport and cell cycle regulation. DDX3X also partakes in HIV-1 replication and hepatitis C viral infections. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 2mg/ml |
| Alternative Names | ATP dependent RNA helicase DDX3X antibody ATP-dependent RNA helicase DDX3X antibody CAP Rf antibody DBX antibody DDX14 antibody DDX3X antibody DDX3X_HUMAN antibody DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody DEAD (Asp-Glu-Ala-Asp) box helicase 3 X-linked antibody DEAD box antibody DEAD box protein 3 antibody DEAD box protein 3 X-chromosomal antibody DEAD box X isoform antibody DEAD box X isoform antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody DEAD/H box 3 antibody DEAD/H box 3 X-linked antibody Helicase like protein 2 antibody Helicase-like protein 2 antibody HLP2 antibody X isoform antibody X-chromosomal antibody |
|---|---|
| Molecular Weight(MW) | 73/71 kDa |
| Cellular Localization | Nucleus speckle. Cytoplasm. |
Application
Fig1: Western blot analysis of DDX3X on human DDX3X recombinant protein using anti-DDX3X antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DDX3X on HEK293 (1) and DDX3X-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX3X antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of DDX3X on different cell lysate using anti-DDX3X antibody at 1/1,000 dilution. Positive control： Lane1: Hela Lane2: NIH3T3 Lane3: C6 Lane4: COS7 Lane5: A431 Lane6: HEK293
Application
Fig4:ICC staining DDX3X (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DDX3X antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-DDX3X antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with DDX3X antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Application
Fig8:Flow cytometric analysis of HepG2 cells with DDX3X antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human DDX3X recombinant protein, DDX3X-hIgGFc transfected HEK293 cell lysate, Hela, NIH3T3, C6, COS7, A431, HEK293, HepG2, human cervical cancer tissue, human ovarian cancer tissue. |
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| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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