| 存储条件 |
|---|
| Product Name | RF1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cl1 protein antibody D5S1995 antibody ERF antibody eRF1 antibody ERF1_HUMAN antibody ETF1 antibody Eukaryotic peptide chain release factor subunit 1 antibody Eukaryotic release factor 1 antibody Eukaryotic translation termination factor 1 antibody MGC111066 antibody Polypeptide chain release factor 1 antibody Protein Cl1 antibody RF1 antibody Sup45 (yeast omnipotent suppressor 45) homolog like 1 antibody SUP45L1 antibody TB3 1 antibody TB3-1 antibody |
| Product description | Translation is carried out by the ribosome and several associated protein factors through three consecutive steps: initiation, elongation and termination. Termination of protein synthesis takes place when the ribosomal A site is occupied simultaneously by one of three stop codons and by a class 1 translation termination factor. In eukaryotes, this termination factor is the eukaryotic release factor 1 (eRF1), a protein that promotes hydrolysis of the last peptidyl-tRNA on the ribosome. eRF1 activity is stimulated by the association with the GTP-binding protein eRF3. eRF1 forms a quaternary complex with eRF3, GTP and the ribosome. This complex performs a dual role, where, in the "GTP state," it controls the positioning of eRF1 toward the stop codon and peptidyl-tRNA, and, in the "GDP state," it promotes the release of the eRFs from the ribosome. eRF1 contains a highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide, which is essential for the interaction of eRF1 with the ribosome. The gene encoding human eRF1 maps to chromosome 5q31.2. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Cl1 protein antibody D5S1995 antibody ERF antibody eRF1 antibody ERF1_HUMAN antibody ETF1 antibody Eukaryotic peptide chain release factor subunit 1 antibody Eukaryotic release factor 1 antibody Eukaryotic translation termination factor 1 antibody MGC111066 antibody Polypeptide chain release factor 1 antibody Protein Cl1 antibody RF1 antibody Sup45 (yeast omnipotent suppressor 45) homolog like 1 antibody SUP45L1 antibody TB3 1 antibody TB3-1 antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P62495 |
|---|
Application
Fig1: Western blot analysis of eRF1 on human eRF1 recombinant protein using anti-eRF1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of eRF1 on HEK293 (1) and eRF1-hIgGFc transfected HEK293 (2) cell lysate using anti-eRF1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of eRF1 on different cell lysate using anti-eRF1 antibody at 1/1,000 dilution. Positive control： Lane1: MCF-7 Lane2: T47D Lane3: MOLT4 Lane4: Raji
Application
Fig4:Flow cytometric analysis of Hela cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Application
Fig5: Flow cytometric analysis of HepG2 cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human eRF1 recombinant protein, eRF1-hIgGFc transfected HEK293 cell lysate, MCF-7, T47D, MOLT4, Raji, Hela, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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