| 存储条件 |
|---|
| Product Name | PTPRC [C11-C10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B220 antibody CD 45 antibody CD45 antibody CD45 antigen antibody CD45R antibody GP180 antibody L-CA antibody LCA antibody Leukocyte common antigen antibody LY5 antibody Lyt4 antibody Protein tyrosine phosphatase receptor type C antibody Protein tyrosine phosphatase, receptor type, c polypeptide antibody Ptprc antibody PTPRC_HUMAN antibody Receptor-type tyrosine-protein phosphatase C antibody T200 antibody T200 glycoprotein antibody T200 leukocyte common antigen antibody |
| Product description | CD45 has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45 are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5 and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45, while the intracellular domain is conserved. CD45 functions as a phosphotyrosine phosphatase, a vital component for efficient tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45 is contained within the conserved intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several members of these two families, including Lck, Fyn and ZAP-70, have been implicated as physiological substrates of CD45. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:250~1:1000 IHC:1:20~1:100 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | B220 antibody CD 45 antibody CD45 antibody CD45 antigen antibody CD45R antibody GP180 antibody L-CA antibody LCA antibody Leukocyte common antigen antibody LY5 antibody Lyt4 antibody Protein tyrosine phosphatase receptor type C antibody Protein tyrosine phosphatase receptor type c polypeptide antibody Ptprc antibody PTPRC_HUMAN antibody Receptor-type tyrosine-protein phosphatase C antibody T200 antibody T200 glycoprotein antibody T200 leukocyte common antigen antibody |
|---|---|
| Molecular Weight(MW) | 147 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P08575 |
|---|
Application
Fig1: Western blot analysis of PTPRC on human PTPRC recombinant protein using anti-PTPRC antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PTPRC on HEK293 (1) and PTPRC-hIgGFc transfected HEK293 (2) cell lysate using anti-PTPRC antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PTPRC on Hela and A431 cell lysate using anti-PTPRC antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTPRC antibody. Counter stained with hematoxylin.| Positive Control | Human PTPRC recombinant protein, PTPRC-hIgGFc transfected HEK293 cell lysate, Hela, A431 cell lysate, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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