| 存储条件 |
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| Product Name | GRM7 [D3-G8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ40498 antibody GLUR7 antibody Glutamate receptor metabotropic 7 antibody GPRC1G antibody GRM7 antibody GRM7_HUMAN antibody Metabotropic glutamate receptor 7 antibody MGLU7 antibody mGluR7 antibody OTTHUMP00000206961 antibody OTTHUMP00000214674 antibody OTTHUMP00000214675 antibody |
| Product description | L-glutamate is the major excitatory neurotransmitter in the central nervous system, and it activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into three groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5, and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3, while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | FLJ40498 antibody GLUR7 antibody Glutamate receptor metabotropic 7 antibody GPRC1G antibody GRM7 antibody GRM7_HUMAN antibody Metabotropic glutamate receptor 7 antibody MGLU7 antibody mGluR7 antibody OTTHUMP00000206961 antibody OTTHUMP00000214674 antibody OTTHUMP00000214675 antibody |
|---|---|
| Molecular Weight(MW) | 102 kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | Q14831 |
|---|
Application
Fig1: Western blot analysis of GRM7 on human GRM7 recombinant protein using anti-GRM7 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of GRM7 on HEK293 (1) and GRM7-hIgGFc transfected HEK293 (2) cell lysate using anti-GRM7 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining GRM7 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining GRM7 (green) and Actin filaments (red) in SMMC-7721 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of SH-SY5Y cells with GRM7 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human GRM7 recombinant protein, GRM7-hIgGFc transfected HEK293 cell lysate, Hela, SMMC-7721, SH-SY5Y. |
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| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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