| 存储条件 |
|---|
| Product Name | Moesin [SC69-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Epididymis luminal protein 70 antibody HEL70 antibody Membrane organizing extension spike protein antibody Membrane-organizing extension spike protein antibody MOES_HUMAN antibody Moesin antibody Moesin/anaplastic lymphoma kinase fusion protein antibody Msn antibody MSN/ALK fusion antibody |
| Product description | Ezrin, Moesin and Radixin belong to a family of highly homologous Actin-associated proteins that are localized just beneath the plasma membrane. These proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share more than 70% homology with Ezrin and are co-expressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Epididymis luminal protein 70 antibody HEL70 antibody Membrane organizing extension spike protein antibody Membrane-organizing extension spike protein antibody MOES_HUMAN antibody Moesin antibody Moesin/anaplastic lymphoma kinase fusion protein antibody Msn antibody MSN/ALK fusion antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa |
| Cellular Localization | Cell membrane, Cytoplasm, Apical cell membrane. |
Application
Fig1: Western blot analysis of Moesin on different lysates using anti-Moesin antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: SH-SY-5Y
Application
Fig2: ICC staining Moesin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Moesin in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Moesin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Moesin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Moesin antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Moesin antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Moesin antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Moesin antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Hela cells with Moesin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | NCCIT, Raji, Hela, SH-SY-5Y, human tonsil tissue, mouse placenta tissue, human spleen tissue, mouse stomach tissue, human breast carcinoma tissue, mouse lung tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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