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| Product Name | nNOS [ST520] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | 2310005C01Rik antibody BNOS antibody Constitutive NOS antibody EC 1.14.13.39 antibody IHPS 1 antibody IHPS1 antibody N-NOS antibody NC-NOS antibody neuronal Nitric Oxide Synthase antibody Neuronal NOS antibody Nitric oxide synthase , neuronal, included antibody Nitric oxide synthase 1 (neuronal) antibody Nitric oxide synthase 1 antibody Nitric oxide synthase, brain antibody Nitric oxide synthase, penile neuronal, included antibody NNOS antibody NO antibody NOS 1 antibody NOS antibody NOS type I antibody NOS-I antibody NOS1 antibody NOS1_HUMAN antibody Peptidyl-cysteine S-nitrosylase NOS1 antibody |
| Product description | Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 2310005C01Rik antibody BNOS antibody Constitutive NOS antibody EC 1.14.13.39 antibody IHPS 1 antibody IHPS1 antibody N-NOS antibody NC-NOS antibody neuronal Nitric Oxide Synthase antibody Neuronal NOS antibody Nitric oxide synthase neuronal included antibody Nitric oxide synthase 1 (neuronal) antibody Nitric oxide synthase 1 antibody Nitric oxide synthase brain antibody Nitric oxide synthase penile neuronal included antibody NNOS antibody NO antibody NOS 1 antibody NOS antibody NOS type I antibody NOS-I antibody NOS1 antibody NOS1_HUMAN antibody Peptidyl-cysteine S-nitrosylase NOS1 antibody |
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| Molecular Weight(MW) | 161 kDa |
| Cellular Localization | Cell membrane, Cell projection. |
Application
Fig1: Western blot analysis of nNOS on mouse heart lysates using anti-nNOS antibody at 1/1,000 dilution.
Application
Fig2: ICC staining nNOS in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining nNOS in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining nNOS in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-nNOS antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-nNOS antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-nNOS antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-nNOS antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of PC-12 cells with nNOS antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | PC-12, PC-3M, SH-SY-5Y, mouse heart tissue, rat testis tissue, rat brain tissue, mouse brain tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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