| 存储条件 |
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| Product Name | Phospho-FOXO3a(S253) [ST49-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AF6q21 antibody AF6q21 protein antibody DKFZp781A0677 antibody FKHR2 antibody FKHRL 1 antibody FKHRL1 antibody FKHRL1P2 antibody Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody Forkhead box O3 antibody Forkhead box O3A antibody Forkhead box protein O3 antibody Forkhead box protein O3A antibody Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody Forkhead homolog (rhabdomyosarcoma) like 1 antibody Forkhead in rhabdomyosarcoma like 1 antibody Forkhead in rhabdomyosarcoma-like 1 antibody FOX O3A antibody FOXO2 antibody foxo3 antibody FOXO3_HUMAN antibody FOXO3A antibody MGC12739 antibody MGC31925 antibody |
| Product description | FKHRL1 (for forkhead in rhabdomyosarcoma) is a member of the FKHR subfamily of forkhead transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 at threonine-32 and serine-253. Phosphorylation by Akt1 negatively regulates FKHRL1 by promoting its export from the nucleus. Phosphorylated FKHRL1 associates with 14-3-3 proteins and this complex is retained in the cytoplasm. Growth factor withdrawal stimulates FKHRL1 dephosphorylation and nuclear translocation, leading to FKHR-induced gene-specific transcriptional activation. Within the nucleus, dephospho-rylated FKHRL1 triggers apoptosis by inducing the expression of genes that are critical for cell death. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser253 of human FOXO3a. |
| Modification | p-S253 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:1,000-1:5,000 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | AF6q21 antibody AF6q21 protein antibody DKFZp781A0677 antibody FKHR2 antibody FKHRL 1 antibody FKHRL1 antibody FKHRL1P2 antibody Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody Forkhead box O3 antibody Forkhead box O3A antibody Forkhead box protein O3 antibody Forkhead box protein O3A antibody Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody Forkhead homolog (rhabdomyosarcoma) like 1 antibody Forkhead in rhabdomyosarcoma like 1 antibody Forkhead in rhabdomyosarcoma-like 1 antibody FOX O3A antibody FOXO2 antibody foxo3 antibody FOXO3_HUMAN antibody FOXO3A antibody MGC12739 antibody MGC31925 antibody |
|---|---|
| Molecular Weight(MW) | 71 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Phospho-FOXO3a(S253) on different lysates using anti-Phospho-FOXO3a(S253) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC12
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-FOXO3a(S253) antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-FOXO3a(S253) antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-FOXO3a(S253) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-FOXO3a(S253) antibody. Counter stained with hematoxylin.| Positive Control | PC12, Hela, human tonsil tissue, human breast carcinoma tissue, human pancreas tissue, mouse pancreas tissue. |
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| Application Notes | WB:1:1,000-1:5,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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