| 存储条件 |
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| Product Name | beta Arrestin 1 [ST51-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARB1 antibody ARR1 antibody ARRB1 antibody ARRB1_HUMAN antibody Arrestin 2 antibody Arrestin beta 1 antibody Arrestin beta-1 antibody Beta-arrestin-1 antibody |
| Product description | The members of the G protein coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. Members of theβ-Arrestin family regulate receptor binding to G proteins. β-Arrestins have been found to be located at postsynaptic sites, where they are thought to act in concert withβARK (βARK1, also designated GRK 2, orβARK2, also designated GRK 3 to regulate G protein-coupled neurotransmitter receptors. Expression ofβ-Arrestin-1 and b-Arrestin-2 is seen predominantly in spleen and neuronal tissues. It has been shown thatβ-Arrestin-1 expression is modulated by intracellular cAMP, which may be a novel mechanism for the regulation of receptor-mediated responses. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ARB1 antibody ARR1 antibody ARRB1 antibody ARRB1_HUMAN antibody Arrestin 2 antibody Arrestin beta 1 antibody Arrestin beta-1 antibody Beta-arrestin-1 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell membrane, Membrane. |
Application
Fig1: Western blot analysis of beta Arrestin 1 on different lysates using anti-beta Arrestin 1 antibody at 1/1,000 dilution. Positive control: Lane 1: PC12 Lane 2: Jurkat
Application
Fig2: ICC staining beta Arrestin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining beta Arrestin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining beta Arrestin 1 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Hela cells with beta Arrestin 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, PC12, Hela, Jurkat, mouse lung tissue, human lung tissue, mouse brain tissue, human liver cancer tissue, human breast carcinoma tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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