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| Product Name | Topoisomerase Ⅱ alpha [SY27-00] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | alpha isozyme antibody ATP hydrolyzing DNA topoisomerase II alfa antibody DNA gyrase antibody DNA topoisomerase (ATP hydrolyzing) antibody DNA topoisomerase 2 alpha antibody DNA topoisomerase 2-alpha antibody DNA topoisomerase II 170 kD antibody DNA topoisomerase II alpha isozyme antibody DNA topoisomerase II antibody DNA Topoisomerase2 antibody TOP 2A antibody TOP2 antibody TOP2A antibody TOP2A_HUMAN antibody Topoisomerase DNA II alpha 170kDa antibody TP2A antibody |
| Product description | DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination, and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIα and Topo IIβ. Topo IIβ and Topo IIα, are largely homologous at their N-terminal three quarters, however, the C-terminal segments are considerably divergent, suggesting that these regions may mediate different cellular functions and account for the observed differential tissue expression patterns of the two isoforms. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:1,000-1:2,000 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | alpha isozyme antibody ATP hydrolyzing DNA topoisomerase II alfa antibody DNA gyrase antibody DNA topoisomerase (ATP hydrolyzing) antibody DNA topoisomerase 2 alpha antibody DNA topoisomerase 2-alpha antibody DNA topoisomerase II 170 kD antibody DNA topoisomerase II alpha isozyme antibody DNA topoisomerase II antibody DNA Topoisomerase2 antibody TOP 2A antibody TOP2 antibody TOP2A antibody TOP2A_HUMAN antibody Topoisomerase DNA II alpha 170kDa antibody TP2A antibody |
|---|---|
| Molecular Weight(MW) | 174 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Topoisomerase Ⅱ alpha on mouse testis lysates using anti-Topoisomerase Ⅱ alpha antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Topoisomerase Ⅱ alpha antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Topoisomerase Ⅱ alpha antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Topoisomerase Ⅱ alpha antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Topoisomerase Ⅱ alpha antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Topoisomerase Ⅱ alpha antibody. Counter stained with hematoxylin.| Positive Control | Human tonsil tissue, human kidney tissue, mouse testis tissue, mouse colon tissue, mouse kidney tissue. |
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| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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