For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application
Dilution
Western Blotting
1:1000
Simple Western™
1:50 - 1:250
Immunoprecipitation
1:50
Immunohistochemistry (Paraffin)
1:800 - 1:3200
Immunofluorescence (Immunocytochemistry)
1:800 - 1:1600
Flow Cytometry (Fixed/Permeabilized)
1:800 - 1:1600
Chromatin IP
1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #86124.
Specificity / Sensitivity
HA-Tag (C29F4) Rabbit Monoclonal Antibody detects exogenously expressed proteins containing the HA epitope tag. This antibody may cross-react with a protein of unknown origin ~100kDa.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA).
