Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Phospho-MARCKS (Ser159/163) (D13D2) Rabbit Monoclonal Antibody recognizes endogenous levels of MARCKS protein when phosphorylated at Ser159 and Ser163 (human residues).
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology:
Monkey, Chicken, Xenopus, Zebrafish, Bovine, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser159 and Ser163 of human MARCKS protein.