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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb

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包装规格 交货周期 质量标准 目录价 会员专享价 数量
20 µl 现货2-3天 原装正品
- +
基础信息
存储条件 -20℃
产品详情

Product Usage Information

ApplicationDilution
Western Blotting1:2000
Fluorescent Western1:2000
Simple Western™1:10 - 1:50
Immunoprecipitation1:50
IHC Leica Bond1:400 - 1:1600
Immunohistochemistry (Paraffin)1:200 - 1:800
Immunofluorescence (Immunocytochemistry)1:200 - 1:400
Flow Cytometry (Fixed/Permeabilized)1:800 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #
45899.

Specificity / Sensitivity

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit Monoclonal Antibody detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish, Bovine, Dog, Pig, S. cerevisiae

Species predicted to react based on 100% sequence homology: 

Chicken, C. elegans

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.  


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