Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
AMPK alpha (D5A2) Rabbit Monoclonal Antibody detects endogenous levels of AMPKα protein. This antibody detects both the α1 and α2 isoforms of the catalytic subunit.
Species Reactivity:
Human, Mouse, Rat, Monkey, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg21 of human AMPKα.
