For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application
Dilution
Western Blotting
1:1000
Simple Western™
1:10 - 1:50
Immunoprecipitation
1:50
Immunohistochemistry (Paraffin)
1:400 - 1:1600
Immunofluorescence (Immunocytochemistry)
1:3200 - 1:6400
Chromatin IP
1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #48277.
Specificity / Sensitivity
BRM (D9E8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total BRM protein. This antibody does not cross-react with BRG1 protein.
Species Reactivity:
Human, Monkey
Species predicted to react based on 100% sequence homology:
Dog
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly264 of human BRM protein.
