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BRD4 (E2A7X) Rabbit mAb

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包装规格 交货周期 质量标准 目录价 会员专享价 数量
100 µl 现货2-3天 原装正品
- +
基础信息
存储条件 -20℃
产品详情

Product Usage Information

For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.


The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.


The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.

ApplicationDilution
Western Blotting1:1000
Immunoprecipitation1:50
Immunohistochemistry (Paraffin)1:20000 - 1:80000
Chromatin IP1:50
Chromatin IP-seq1:50
CUT&RUN1:50
CUT&Tag1:50
eCLIP1:200
For more information about the RBP-eCLIP service please visit Eclipsebio.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #
35632.

Specificity / Sensitivity

BRD4 (E2A7X) Rabbit Monoclonal Antibody recognizes endogenous levels of total BRD4 protein. This antibody specifically recognizes the BRD4 long isoform (UniProt #O60885-1) and does not recognize other BRD4 isoforms.

Species Reactivity:

Human

Species predicted to react based on 100% sequence homology: 

Bovine, Dog, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1129 of human BRD4 protein.


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