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Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb

产品编号 : 8814S

英文名称 :

中文名称 : 抗体

品牌 : CST

包装规格	交货周期	质量标准	目录价	会员专享价	数量
100 µl	现货2-3天	原装正品	￥5220	登录后可见	- +

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基础信息产品详情相关文献

基础信息

存储条件	-20℃

产品详情

Product Usage Information

For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:400 - 1:1600
Immunofluorescence (Immunocytochemistry)	1:800 - 1:3200
Flow Cytometry (Fixed/Permeabilized)	1:800 - 1:3200
Chromatin IP	1:100
Chromatin IP-seq	1:100
CUT&RUN	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #33893.

Specificity / Sensitivity

Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit Monoclonal Antibody recognizes endogenous β-catenin protein when residues Ser33, Ser37, and Thr41 are not phosphorylated. This antibody does not detect β-catenin protein if tri-phosphorylated at Ser33/Ser37/Thr41. This antibody may also detect β-catenin protein when singly phosphorylated at Ser33. This specificity data was derived from competition ELISA and dot blot analysis using synthetic peptides.

Species Reactivity:

Human, Mouse, Rat, Monkey

Species predicted to react based on 100% sequence homology:

Chicken, Xenopus, Zebrafish, Bovine, Dog, Pig, Horse, Guinea Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human β-catenin protein.

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