| 存储条件 |
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| Product Name | ROCK2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within C terminal Human ROCK2. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:1000 ICC/IF:1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ROCK2 |
|---|---|
| Gene Synonyms | ROCK-II |
| Gene Full Name | Rho associated coiled-coil containing protein kinase 2 |
| Gene Summary | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008] |
| Alternative Names | coiled-coil-containing protein kinase 2 antibody KIAA0619 antibody p164 ROCK 2 antibody p164 ROCK-2 antibody Rho associated coiled coil containing protein kinase 2 antibody Rho associated protein kinase 2 antibody Rho associated coiled coil containing protein kinase II antibody Rho kinase 2 antibody Rho-associated antibody Rho-associated protein kinase 2 antibody coiled-coil-containing protein kinase 2 antibody KIAA0619 antibody p164 ROCK 2 antibody p164 ROCK-2 antibody Rho associated coiled coil containing protein kinase 2 antibody Rho associated protein kinase 2 antibody Rho associated coiled coil containing protein kinase II antibody Rho kinase 2 antibody Rho-associated antibody Rho-associated protein kinase 2 antibody ROCK 2 antibody Rock II antibody Rock2 antibody ROCK2_HUMAN antibody Rock2m antibody ROK alpha antibody ROKalpha antibody |
| Molecular Weight(MW) | 161kDa(Observed band size: 150kDa) |
| Cellular Localization | Cytoplasm, Cell membrane, Nucleus, cytoskeleton, microtubule organizing center, centrosome. |
WB
Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: MCF7 cell lysate, Lane 3: C2C12 cell lysate, Lane 4: C6 cell lysate, Lane 5: Mouse brain tissue lysate, Lane 6: Rat brain tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ROCK2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling ROCK2 with Rabbit anti-ROCK2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ROCK2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 IHC:1:1000 ICC/IF:1:100 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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