| 存储条件 |
|---|
| Product Name | CD11b Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human CD11b aa 1-100. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:2000 IHC:1:5000-1:20000 IF-P:1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ITGAM |
|---|---|
| Gene Synonyms | CR3A MO1A CD11B MAC-1 MAC1A SLEB6 |
| Gene Full Name | integrin subunit alpha M |
| Gene Summary | This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Alternative Names | antigen CD11b (p170) antibody Antigen CD11b p170 antibody CD11 antigen like family member B antibody CD11 antigen-like family member B antibody CD11b antibody CD11b/CD18 antibody CD49d antibody Cell surface glycoprotein MAC-1 subunit alpha antibody Complement component 3 receptor 3 subunit antibody Complement Component Receptor 3 Alpha antibody antigen CD11b (p170) antibody Antigen CD11b p170 antibody CD11 antigen like family member B antibody CD11 antigen-like family member B antibody CD11b antibody CD11b/CD18 antibody CD49d antibody Cell surface glycoprotein MAC-1 subunit alpha antibody Complement component 3 receptor 3 subunit antibody Complement Component Receptor 3 Alpha antibody Complement receptor type 3 alpha subunit antibody CR 3 alpha chain (CR3A) antibody CR 3 alpha chain antibody CR-3 alpha chain antibody CR3 antibody CR3A antibody F730045J24Rik antibody Integrin Alpha M antibody Integrin alpha M chain antibody Integrin alpha-M antibody Integrin beta 2 alpha subunit antibody Integrin subunit alpha M antibody integrin alpha M (complement component 3 receptor 3 subunit) antibody ITAM_HUMAN antibody ITGAM antibody Leukocyte adhesion receptor MO1 antibody Ly-40 antibody MAC 1 antibody Mac-1a antibody MAC1 antibody Mac1 alpha subunit antibody MAC1A antibody Macrophage antigen alpha polypeptide antibody MGC117044 antibody Mo1 alpha subunit antibody MO1A antibody Neutrophil adherence receptor alpha M subunit antibody Neutrophil adherence receptor antibody SLEB6 antibody |
| Molecular Weight(MW) | 127 kDa(Observed band size: 170kDa) |
| Cellular Localization | Cell membrane, Membrane raft. |
WB
Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody at 1/2,000 dilution. Lane 1: TF-1 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (15 µg/Lane) Lane 3: U-937 cell lysate (30 µg/Lane) Lane 4: Jurkat cell lysate (negative) (10 µg/Lane) Lane 5: RAW264.7 cell lysate (5 µg/Lane) Lane 6: J774A.1 cell lysate (5 µg/Lane) Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD11b antibody at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling CD11b with Rabbit anti-CD11b antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD11b antibody at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD11b antibody at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IHC:1:5000-1:20000 IF-P:1:1000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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