| 存储条件 |
|---|
| Product Name | KDM6A Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human KDM6A aa 60-800 / 1,401. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:4000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KDM6A |
|---|---|
| Gene Synonyms | UTX KABUK2 bA386N14.2 |
| Gene Full Name | lysine demethylase 6A |
| Gene Summary | This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014] |
| Alternative Names | bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX) antibody bA386N14.2 antibody bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX antibody DKFZp686A03225 antibody Histone demethylase UTX antibody KABUK2 antibody Kdm6a antibody KDM6A_HUMAN antibody Lysine (K) specific demethylase 6A antibody Lysine K specific demethylase 6A antibody bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX) antibody bA386N14.2 antibody bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX antibody DKFZp686A03225 antibody Histone demethylase UTX antibody KABUK2 antibody Kdm6a antibody KDM6A_HUMAN antibody Lysine (K) specific demethylase 6A antibody Lysine K specific demethylase 6A antibody Lysine specific demethylase 6A ubiquitously transcribed tetratricopeptide repeat gene X chromosome antibody Lysine-specific demethylase 6A antibody MGC141941 antibody Ubiquitously transcribed tetratricopeptide repeat X chromosome antibody Ubiquitously transcribed tetratricopeptide repeat protein X linked antibody Ubiquitously transcribed TPR gene on the X chromosome antibody Ubiquitously transcribed TPR protein on the X chromosome antibody Ubiquitously transcribed X chromosome tetratricopeptide repeat protein antibody Ubiquitously-transcribed TPR protein on the X chromosome antibody Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein antibody UTX antibody |
| Molecular Weight(MW) | 154kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of KDM6A on different lysates with Rabbit anti-KDM6A antibody at 1/1,000 dilution. Lane 1: Hela cell lysate, Lane 2: NIH/3T3 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-KDM6A antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-KDM6A antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:4000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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