| 存储条件 |
|---|
| Product Name | Rabbit polyclonal antibody to PER1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human PER1 recombinant protein (Position: D1119-M1200). Human PER1 shares 96.3% and 95.1% amino acid (aa) sequence identity with mouse and rat PER1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PER1 |
|---|---|
| Gene Synonyms | PER hPER RIGUI |
| Gene Full Name | period circadian regulator 1 |
| Gene Summary | This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described. [provided by RefSeq, Jan 2014] |
| Alternative Names | hPER hPER1 KIAA0482 PER PER1 RIGUI |
| Molecular Weight(MW) | 136kDa(Observed MW 200kDa) |
| Cellular Localization | Nucleus,Cytoplasm. |
WB
Western blot analysis of PER1 using anti-PER1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Hepa1/6 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PER1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PER1 using anti-PER1 antibody. PER1 was detected in a paraffin-embedded section of human gastric cancer tissue. The tissue section was incubated with rabbit anti-PER1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of PER1 using anti-PER1 antibody. PER1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-PER1 Antibody at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)was used as secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 0.5mg/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
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