| 存储条件 |
|---|
| Product Name | NMDAR1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human NMDAR1 aa 870-910. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-FIF-PIHCWB |
| WB:1:1000-1:5000 IHC:1:1000 IF-F:1:200 IF-P:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GRIN1 |
|---|---|
| Gene Synonyms | NR1 MRD8 hNR1 GluN1 NMDA1 DEE101 NDHMSD NDHMSR NMD-R1 NMDAR1 |
| Gene Full Name | glutamate ionotropic receptor NMDA type subunit 1 |
| Gene Summary | The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| Alternative Names | GluN1 antibody Glutamate [NMDA] receptor subunit zeta-1 antibody Glutamate receptor ionotropic N methyl D aspartate 1 antibody Glutamate receptor ionotropic N-methyl-D aspartate subunit 1 antibody glutamate receptor ionotropic NMDA 1 antibody Grin1 antibody MRD8 antibody N methyl D aspartate receptor antibody N methyl D aspartate receptor channel subunit zeta 1 antibody N methyl D aspartate receptor subunit NR1 antibody GluN1 antibody Glutamate [NMDA] receptor subunit zeta-1 antibody Glutamate receptor ionotropic N methyl D aspartate 1 antibody Glutamate receptor ionotropic N-methyl-D aspartate subunit 1 antibody glutamate receptor ionotropic NMDA 1 antibody Grin1 antibody MRD8 antibody N methyl D aspartate receptor antibody N methyl D aspartate receptor channel subunit zeta 1 antibody N methyl D aspartate receptor subunit NR1 antibody N-methyl-D-aspartate receptor subunit NR1 antibody NMD-R1 antibody NMDA 1 antibody NMDA R1 antibody NMDA receptor 1 antibody NMDA1 antibody NMDAR antibody NMDZ1_HUMAN antibody NR1 antibody |
| Molecular Weight(MW) | 105kDa(Observed band size: 120kDa) |
| Cellular Localization | Cell membrane, postsynaptic cell membrane, postsynaptic density. |
WB
Western blot analysis of NMDAR1 on different lysates with Rabbit anti-NMDAR1 antibody at 1/5,000 dilution. Lane 1: MCF7 cell lysate (15 µg/Lane) Lane 2: Human brain tissue lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Lane 5: Mouse heart tissue lysate (negative) (20 µg/Lane) Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane) Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-NMDAR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Immunofluorescence analysis of frozen mouse hippocampus tissue with Rabbit anti-NMDAR1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IF-P
Immunofluorescence analysis of frozen mouse hippocampus tissue with Rabbit anti-NMDAR1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
FC
Flow cytometric analysis of NMDAR1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000-1:5000 IHC:1:1000 IF-F:1:200 IF-P:1:50-1:200 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
