| 存储条件 |
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| Product Name | ACSL4/FACL4 Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human FACL4 aa 561-711 (Cytoplasmic). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ACSL4 |
|---|---|
| Gene Synonyms | ACS4 FACL4 LACS4 MRX63 MRX68 XLID63 |
| Gene Full Name | acyl-CoA synthetase long chain family member 4 |
| Gene Summary | The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016] |
| Alternative Names | ACS 4 antibody ACS4 antibody ACSL 4 antibody Acsl4 antibody ACSL4_HUMAN antibody acyl CoA synthetase 4 antibody Acyl CoA synthetase long chain family member 4 antibody FACL 4 antibody FACL4 antibody Fatty acid Coenzyme A ligase antibody ACS 4 antibody ACS4 antibody ACSL 4 antibody Acsl4 antibody ACSL4_HUMAN antibody acyl CoA synthetase 4 antibody Acyl CoA synthetase long chain family member 4 antibody FACL 4 antibody FACL4 antibody Fatty acid Coenzyme A ligase antibody fatty acid Coenzyme A ligase long-chain 4 antibody LACS 4 antibody LACS4 antibody Lignoceroyl CoA synthase antibody Long chain 4 antibody long chain acyl CoA synthetase 4 antibody long chain fatty acid CoA ligase 4 antibody long chain fatty acid Coenzyme A ligase 4 antibody Long-chain acyl-CoA synthetase 4 antibody Long-chain-fatty-acid--CoA ligase 4 antibody MRX63 antibody MRX68 antibody |
| Molecular Weight(MW) | 79kDa(Observed band size: 75kDa) |
| Cellular Localization | Peroxisome membrane, Mitochondrion outer membrane, Microsome membrane, Endoplasmic reticulum membrane, Cell membrane. |
WB
Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody at 1/1,000 dilution. Lane 1: PC-12 cell lysate, Lane 2: C6 cell lysate, Lane 3: L6 cell lysate, Lane 4: Rat brain tissue lysate, Lane 5: Rat lung tissue lysate, Lane 6: Rat thymus tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: Mouse brain tissue lysate (40 µg/Lane), Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACSL4 / FACL4 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACSL4 / FACL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000-1:5000 IHC:1:1000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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