| 存储条件 |
|---|
| Product Name | gamma Tubulin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Tubulin gamma-2 chain aa 227-407 / 451. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:2000 IHC:1:200 ICC:1:100 FC:1:50-1:100 IP:Use at an assay dependent concentration. | |
| Species Reactivity | ChickenCowDogFishHamsterHumanMonkeyMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TUBG1 |
|---|---|
| Gene Synonyms | TUBG GCP-1 CDCBM4 TUBGCP1 |
| Gene Full Name | tubulin gamma 1 |
| Gene Summary | This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009] |
| Alternative Names | Gamma 1 tubulin antibody Gamma 2 tubulin antibody Gamma Tubulin 1 antibody Gamma Tubulin 2 antibody Gamma tubulin complex component 1 antibody Gamma-2-tubulin antibody GCP 1 antibody GCP1 antibody MGC131994 antibody TBG2_HUMAN antibody Gamma 1 tubulin antibody Gamma 2 tubulin antibody Gamma Tubulin 1 antibody Gamma Tubulin 2 antibody Gamma tubulin complex component 1 antibody Gamma-2-tubulin antibody GCP 1 antibody GCP1 antibody MGC131994 antibody TBG2_HUMAN antibody TUBG antibody TUBG1 antibody TUBG2 antibody TUBGCP1 antibody Tubulin gamma 1 chain antibody Tubulin gamma 2 chain antibody Tubulin gamma complex-associated protein 1 antibody Tubulin gamma-2 chain antibody tubulin gamma 1 antibody tubulin gamma 2 antibody tubulin gamma polypeptide antibody Xgam antibody |
| Molecular Weight(MW) | 51kDa(Observed band size: 48kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of gamma Tubulin on different lysates with Rabbit anti-gamma Tubulin antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: COS-1 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 48 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-gamma Tubulin antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling gamma Tubulin with Rabbit anti-gamma Tubulin antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-gamma Tubulin antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of gamma Tubulin was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 IHC:1:200 ICC:1:100 FC:1:50-1:100 IP:Use at an assay dependent concentration. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
