| 存储条件 |
|---|
| Product Name | KPNA6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from Human KPNA6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA6 |
|---|---|
| Gene Synonyms | IPOA7 |
| Gene Full Name | karyopherin subunit alpha 6 |
| Gene Summary | Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family. [provided by RefSeq, Jul 2008] |
| Alternative Names | FLJ11249 IMA7_HUMAN Importin alpha 7 subunit Importin alpha S2 Importin subunit alpha 7 Importin subunit alpha-7 IPOA 7 IPOA7 Karyopherin alpha 6 Karyopherin alpha 6 (importin alpha 7) Karyopherin subunit alpha 6 Karyopherin subunit alpha-6 KPNA 6 KPNA 7 Kpna6 KPNA7 MGC17918 OTTHUMP00000004532 |
| Molecular Weight(MW) | 60kDa(Observed bands:70kDa) |
| Cellular Localization | cytosol,nucleoplasm,nucleus |
WB
Western blot analysis of extracts from Rat brain, using KPNA6 Ab at 1/1000 dilution.
IHC
KPNA6 Ab at 1/100 staining human esophageal cancer by IHC. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.
ICC/IF
KPNA6 Ab staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
抱歉，暂无相关文献
