| 存储条件 |
|---|
| Product Name | IDO Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide derived from the C-terminus of human IDO-1 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:200 ICC:1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IDO1 |
|---|---|
| Gene Synonyms | IDO INDO IDO-1 |
| Gene Full Name | indoleamine 2,3-dioxygenase 1 |
| Gene Summary | This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011] |
| Alternative Names | 3-dioxygenase antibody I23O1_HUMAN antibody IDO 1 antibody IDO antibody IDO-1 antibody IDO1 antibody INDO antibody indolamine 2 3 dioxygenase antibody Indole 2 3 dioxygenase antibody indoleamine 2 3 dioxygenase 1 antibody 3-dioxygenase antibody I23O1_HUMAN antibody IDO 1 antibody IDO antibody IDO-1 antibody IDO1 antibody INDO antibody indolamine 2 3 dioxygenase antibody Indole 2 3 dioxygenase antibody indoleamine 2 3 dioxygenase 1 antibody indoleamine 2 3 dioxygenase antibody Indoleamine 2 3-dioxygenase 1 antibody Indoleamine pyrrole 2 3 dioxygenase antibody Indoleamine-pyrrole 2 antibody |
| Molecular Weight(MW) | 45kDa(Observed band size: 40kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of IDO on different lysates with Rabbit anti-IDO antibody at 1/1,000 dilution. Lane 1: A549 cell lysate, Lane 2: A549 treated with 50ng/mL IFN-gamma for 24 hours cell lysate. Lysates/proteins at 20 µg/Lane. Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-IDO antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells treated with or without 50 ng/mL IFN-gamma for 16 hours labeling IDO with Rabbit anti-IDO antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IDO antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 IHC:1:200 ICC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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