| 存储条件 |
|---|
| Product Name | Lamin B1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADLD antibody lamin B1 antibody Lamin-B1 antibody LMN antibody LMN2 antibody LMNB antibody Lmnb1 antibody LMNB1_HUMAN antibody MGC111419 antibody OTTHUMP00000159218 antibody |
| Product description | A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear Lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type Lamins, such as Lamin B1, undergo a series of modifications, such as farnesylation and phosphorylation. Lamin B1 is a 586 amino acid protein that is encoded by a gene which, when mutated, is involved in the pathogenesis of autosomal dominant adult-onset leukodystrophy (ADLD), a disease characterized by cerebellar dysfunction and symmetric demyelination of the central nervous system. |
| Immunogen | Amino acids 401-490 mapping near the C-terminus of Lamin B1 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ADLD antibody lamin B1 antibody Lamin-B1 antibody LMN antibody LMN2 antibody LMNB antibody Lmnb1 antibody LMNB1_HUMAN antibody MGC111419 antibody OTTHUMP00000159218 antibody |
|---|---|
| Molecular Weight(MW) | 67 kDa |
| Cellular Localization | Nucleus inner membrane |
| SwissProt ID | P20700 |
|---|
Application
Fig1: Western blot analysis of Lamin B1 expression in Jurkat (A) and Y79 (B) whole cell lysates. .
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear envelope and cytoplasmic staining of myocytes (A). Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization (B). .| Positive Control | Hela, Jurkat, Y79, human heart muscle tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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