Intracisternal A-type particles (IAP) are defective endogenous retroviruses that accumulate in the endoplasmic reticulum (ER) of rodent cells. The enveloped particles are produced by assembly and budding of IAP Gag polyproteins at the ER membrane. Readthrough of the terminator UAA occurs between the codons for Met-218 and Ala-219. This particle is a defective retrovirus.
Fig1: ICC staining Retrovirus-related Gag in Retrovirus-related Gag transfected 293 cells (red) and HA-tag staining (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Positive Control
Retrovirus-related Gag protein transfected 293 cells.
Application Notes
ICC:1:50-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
