Anti-Osteoprotegerin抗体
参阅全部 Osteoprotegerin 一抗
兔多克隆抗体to Osteoprotegerin
Rabbit
适用于: WBmore details
与反应: Human
Recombinant fragment corresponding to Human Osteoprotegerin.
Database link: O00300
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab124820
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Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
No preservative, sterile filtered
Immunogen affinity purified
多克隆
unknown
unknown
Abpromise™承诺保证使用ab9986于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 46 kDa). To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOPG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More |
Entrez Gene: 4982 Human
Omim: 602643 Human
SwissProt: O00300 Human
Unigene: 81791 Human
Osteoclastogenesis inhibitory factor antibody
Osteoprotegerin antibody
PDB5 antibody
TNF receptor superfamily member 11b antibody
TNFRSF 11B antibody
TNFRSF11B antibody
TR 1 antibody
TR1 antibody
TR11B_HUMAN antibody
Tumor necrosis factor receptor superfamily member 11B antibody
MGC29565 antibody
OCIF antibody
OPG antibody
Western blot - Anti-Osteoprotegerin antibody (ab9986)
All lanes : Anti-Osteoprotegerin antibody (ab9986) at 1 µg/ml
Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 56 kDawhy is the actual band size different from the predicted?
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
Osteoprotegerin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
