重组人/Murine/大鼠BDNF蛋白(Active)
参阅全部 BDNF 蛋白酶
Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.
The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions.
> 98 % SDS-PAGE.
> 98% pure HPLC analyses.
< 1.000 Eu/µg
Escherichia coli
Full length protein
No
Recombinant
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
27 kDa
129 to 247
aa 129 to 247 refers to the full length mature form. This product does not contain signal peptide or propeptide.
Our Abpromise guarantee covers the use of ab9794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用
HPLC
Cellular Activation
SDS-PAGE
Western blot
Lyophilized
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
复溶
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.
Abrineurin
ANON2
BDNF
BDNF_HUMAN
Brain Derived Neurotrophic Factor
Brain-derived neurotrophic factor
BULN2
MGC34632
Neurotrophin
Western blot - Recombinant Human/Murine/Rat BDNF protein (ab9794)
Rabbit Anti-BDNF antibody at 1 µg/ml + Recombinant Human/Murine/Rat BDNF protein (Active) (ab9794) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 4 minutes
