Anti-hCG抗体
参阅全部 hCG 一抗
兔多克隆抗体to hCG
Rabbit
This antibody reacts with hCG. It stains strongly with the human placenta and placental trophoblasts. No non-specific stainingwith any other tissue has been observed.
适用于: IHC-P, IHC-Fr, WBmore details
与反应: Human
Beta chain of human chorionic gonadotropin.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.3
Protein A purified
多克隆
IgG
Abpromise™承诺保证使用ab9376于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:100-1:500 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Although not required, proteolytic treatment may enhance the immunostaining in overfixed tissues. Read More | |
IHC-Fr | Use at an assay dependent concentration. | |
WB | (1) | Use at an assay dependent concentration. Predicted molecular weight: 18 kDa. |
Entrez Gene: 1081 Human
Entrez Gene: 1082 Human
Omim: 118850 Human
Omim: 118860 Human
SwissProt: P01215 Human
SwissProt: P01233 Human
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choriogonadotropin alpha chain antibody
Chorionic gonadotrophin chain beta antibody
chorionic gonadotrophin subunit alpha antibody
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chorionic gonadotropin, alpha polypeptide antibody
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follicle-stimulating hormone alpha subunit antibody
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FSH Alpha antibody
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glycoprotein hormones, alpha polypeptide antibody
GPHa antibody
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Human Chorionic Gonadotropin antibody
LHA antibody
LSH alpha antibody
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lutropin alpha chain antibody
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thyroid-stimulating hormone alpha chain antibody
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CG alpha antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody (ab9376)
Human normal placenta. Staining is localised extracellular and in the cytoplasm. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab9376 staining hCG in formalin-fixed, paraffin-embedded Human placenta by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody (ab9376)