Anti-alpha 1 Antitrypsin antibody [B9]

• 产品名称

  Anti-alpha 1 Antitrypsin抗体[B9]
  参阅全部 alpha 1 Antitrypsin 一抗

• 描述

  小鼠单克隆抗体[B9] to alpha 1 Antitrypsin

• 宿主

  Mouse

• 经测试应用

  适用于: ELISA, WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length protein corresponding to Human alpha 1 Antitrypsin.
  Database link: P01009

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR286035-1, GR146648-9 and GR146648-10 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.1% Sodium azide

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  B9

• 骨髓瘤

  NS0/1

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9399于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5265 Human

• Omim: 107400 Human

• SwissProt: P01009 Human

• Unigene: 525557 Human

• 别名

• AAT antibody

• Alpha 1 antiproteinase antibody

• Alpha 1 antitrypsin antibody

• Alpha 1 antitrypsin null antibody

• Alpha 1 protease inhibitor antibody

• Alpha-1 protease inhibitor antibody

• Alpha-1-antiproteinase antibody

• alpha1 proteinase inhibitor antibody

• Alpha1AT antibody

• Dom1 antibody

• PI antibody

• PI1 antibody

• PRO0684 antibody

• PRO2275 antibody

• Serine (or cysteine) proteinase inhibitor clade A member 1 antibody

• Serine protease inhibitor 1-1 antibody

• Serine protease inhibitor A1a antibody

• Serpin A1 antibody

• Serpin A1a antibody

• Serpin peptidase inhibitor clade A member 1 antibody

• Serpina1 antibody

• Short peptide from AAT antibody

• SPAAT antibody

• Spi1-1 antibody

• A1A antibody

• A1AT antibody

• A1AT_HUMAN antibody

• 

  Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)

  Western blot of alpha 1 antitrypsin using ab9399 at a concentration of 0.5 µg/ml.
  lane 2 - human plasma, 2 µl
  lane S - standard proteins.
  A - SDS-PAGE, Coomasie staining
  B - Western blot with ab9399.

  Western blot of alpha 1 antitrypsin using ab9399 at a concentration of 0.5 µg/ml.
  lane 2 - human plasma, 2 µl
  lane S - standard proteins.
  A - SDS-PAGE, Coomasie staining
  B - Western blot with ab9399.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)

  Ab9399 staining human normal liver. Staining is localized to the cytoplasm.
  Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• 

  Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)

  Anti-alpha 1 Antitrypsin antibody [B9] (ab9399) at 1 µg/ml + Native human alpha 1 Antitrypsin protein (Active) (ab91136) at 0.1 µg
  
  Secondary
  Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Exposure time: 30 seconds