Anti-IL-1 alpha抗体
参阅全部 IL-1 alpha 一抗
兔多克隆抗体to IL-1 alpha
Rabbit
适用于: ICC/IF, Sandwich ELISA, WB, Neutralising, IHC-P, ELISAmore details
与反应: Human
Recombinant full length protein corresponding to Human IL-1 alpha. Corresponds to Human IL-1 alpha, active form without propeptide. Highly pure (>98%) recombinant hIL-1a (human Interleukin-1-alpha).
Database link: P01583
IHC-P: Human liver tissue; Recombinant human IL-1 alpha protein (ab9615) can be used as a positive control in WB.
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab7632
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C
Shipped at 4°C. Store at -20ºC.
No preservative, sterile filtered
Immunogen affinity purified
多克隆
unknown
unknown
Abpromise™承诺保证使用ab9614于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | Use at an assay dependent concentration. |
| Sandwich ELISA | Use at an assay dependent concentration. To detect IL-1 αlpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271226 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-1 αlpha. Read More | |
| WB | (1) | Use at an assay dependent concentration. To detect hIL-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1a (20 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ELISA | Use at an assay dependent concentration. To detect hIL-1a by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-1a. Read More |
Entrez Gene: 3552 Human
Omim: 147760 Human
SwissProt: P01583 Human
Unigene: 1722 Human
Hematopoietin-1 antibody
IL 1 alpha antibody
IL 1A antibody
IL-1 alpha antibody
Il-1a antibody
IL1 ALPHA antibody
IL1 antibody
IL1A antibody
IL1A_HUMAN antibody
IL1F1 antibody
Interleukin 1 alpha antibody
Interleukin-1 alpha antibody
Interleukin1 alpha antibody
LAF antibody
LEM antibody
Preinterleukin 1 alpha antibody
Pro interleukin 1 alpha antibody
BAF antibody
FAF antibody
Hematopoietin 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody (ab9614)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IL-1 alpha with ab9614 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Western blot - Anti-IL-1 alpha antibody (ab9614)
Sandwich ELISA - Anti-IL-1 alpha antibody (ab9614)
