Anti-TNF alpha抗体[2.1_4E10-1H11]
参阅全部 TNF alpha 一抗
小鼠单克隆抗体[2.1_4E10-1H11] to TNF alpha
Mouse
适用于: Sandwich ELISA, Flow Cyt, IHC-Pmore details
与反应: Human, Recombinant fragment
Recombinant full length protein corresponding to Human TNF alpha. Recombinant Human TNF-a
WB: Recombinant human TNF alpha protein (ab9642). Flow Cyt: THP1 cells.
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1793.
Monoclonal antibodies were produced in BALB/c x C57BL/6 F1 mice
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Lyophilized:Reconstitute with 500µl of sterile water.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
Protein G purified
This antibody was purified from cell culture by Protein G affinity chromatography.
单克隆
2.1_4E10-1H11
IgG1
kappa
Abpromise™承诺保证使用ab9348于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use a concentration of 2 - 4 µg/ml. In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human TNF-α when used with an appropriate biotinylated antigen affinity purified anti-Human TNF-α as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. Read More | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
| IHC-P | Use at an assay dependent concentration. |
Entrez Gene: 7124 Human
Omim: 191160 Human
SwissProt: P01375 Human
Unigene: 241570 Human
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![Sandwich ELISA - Anti-TNF alpha antibody [2.1_4E10-1H11] (ab9348)](https://www.abcam.cn/ps/products/9/ab9348/Images/ab9348-400176-AntiTNF-alpha-antibody.png)
Sandwich ELISA - Anti-TNF alpha antibody [2.1_4E10-1H11] (ab9348)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of Recombinant Human TNF-α when used with suitable detection antibody at a concentration of at least 0.5-1.0 μg/ml.
