Anti-IL-6 antibody [1.2-2B11-2G10]

• 产品名称

  Anti-IL-6抗体[1.2-2B11-2G10]
  参阅全部 IL-6 一抗

• 描述

  小鼠单克隆抗体[1.2-2B11-2G10] to IL-6

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, Sandwich ELISA, WBmore details

• 种属反应性

  与反应: Rat, Human
  

• 免疫原

  Recombinant full length protein corresponding to Human IL-6 aa 29-212. (Peptide available as ab9627).
  Database link: P05231

• 阳性对照

• IHC-P: Human liver tumor and prostate tumor tissues; WB: Human spleen and lung tissue lysates, Rat spleen and lung tissue lysates, Recombinant human IL6 protein (ab9627).

• 常规说明

  Although some customers have been successful in IHC we no longer batch test in this application.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Lyophilized:Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  1.2-2B11-2G10

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab9324于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3569 Human

• Entrez Gene: 24498 Rat

• Omim: 147620 Human

• SwissProt: P05231 Human

• SwissProt: P20607 Rat

• Unigene: 654458 Human

• Unigene: 9873 Rat

• 别名

• B-cell stimulatory factor 2 antibody

• BSF 2 antibody

• BSF-2 antibody

• BSF2 antibody

• CDF antibody

• CTL differentiation factor antibody

• Cytotoxic T cell differentiation factor antibody

• Hepatocyte stimulating factor antibody

• Hepatocyte stimulatory factor antibody

• HGF antibody

• HSF antibody

• Hybridoma growth factor antibody

• Hybridoma growth factor Interferon beta-2 antibody

• Hybridoma plasmacytoma growth factor antibody

• IFN-beta-2 antibody

• IFNB2 antibody

• IL 6 antibody

• IL-6 antibody

• IL6 antibody

• IL6_HUMAN antibody

• Interferon beta 2 antibody

• Interferon beta-2 antibody

• Interleukin 6 antibody

• Interleukin 6 (interferon beta 2) antibody

• Interleukin BSF 2 antibody

• Interleukin-6 antibody

• Interleukin BSF 2 antibody

• B cell differentiation factor antibody

• B cell stimulatory factor 2 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tumor tissue labelling IL-6 with ab9324 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

• 

  Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)

  All lanes : Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) at 1/2000 dilution
  
  Lane 1 : Human Spleen
  Lane 2 : Human Lung
  Lane 3 : Rat Spleen
  Lane 4 : Rat Lung
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : 800CW Goat Anti-Mouse IgG at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Observed band size: 17,25 kDawhy is the actual band size different from the predicted?
  Additional bands at: 50 kDa (possible multimer)
  
  
  
  

  This antibody was raised against an immunogen that is predicted to recognize the glycosylated form of IL6 as well as the IL6delta4 splice variant. The predicted molecular weights are 25 kDa and 17 kDa respectively. The band observed at 50 kDa may represent multimers of IL6 as reported in the literature.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tumor tissue labelling IL-6 with ab9324 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

• 

  Sandwich ELISA - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)

  Assuming 100 µL/well, a concentration of 1.0-2.0 µg/mL of ab9324 will detect at least 1,000 pg/mL of Recombinant Human IL-6 when used with Biotin Anti-IL-6 antibody (ab271236) as the detection antibody at a concentration of approximately 0.10-0.20 µg/mL.