Anti-Androgen Receptor antibody [AR 441]

• 产品名称

  Anti-Androgen Receptor抗体[AR 441]
  参阅全部 Androgen Receptor 一抗

• 描述

  小鼠单克隆抗体[AR 441] to Androgen Receptor

• 宿主

  Mouse

• 特异性

  This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length androgen receptor and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors.

• 经测试应用

  适用于: Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Human
  预测可用于: Macaque monkey

• 免疫原

  Synthetic peptide corresponding to Human Androgen Receptor aa 302-318.
  Sequence:

  STEDTAEYSPFKGGYTK

  
  Database link: P10275

  Run BLAST with Run BLAST with 

• 阳性对照

• Prostate Carcinoma.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 纯度

  Protein G purified

• 纯化说明

  purified immunoglobulin fraction.

• 克隆

  单克隆

• 克隆编号

  AR 441

• 骨髓瘤

  unknown

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9474于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 367 Human

• Omim: 313700 Human

• SwissProt: P10275 Human

• Unigene: 76704 Human

• 形式

  There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.

• 别名

• Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody

• Androgen receptor antibody

• androgen receptor splice variant 4b antibody

• AR antibody

• AR8 antibody

• DHTR antibody

• Dihydro testosterone receptor antibody

• Dihydrotestosterone receptor (DHTR) antibody

• Dihydrotestosterone receptor antibody

• HUMARA antibody

• HYSP1 antibody

• KD antibody

• Kennedy disease (KD) antibody

• NR3C4 antibody

• Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody

• Nuclear receptor subfamily 3 group C member 4 antibody

• SBMA antibody

• SMAX1 antibody

• Spinal and bulbar muscular atrophy (SBMA) antibody

• Spinal and bulbar muscular atrophy antibody

• Testicular Feminization (TFM) antibody

• TFM antibody

• AIS antibody

• ANDR_HUMAN antibody

• Androgen nuclear receptor variant 2 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (ab9474)

  ab9474 staining human prostate by IHC-P.

• 

  Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (ab9474)

  Overlay histogram showing MCF-7 cells stained with ab9474 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (ab9474)

  Overlay histogram showing PC3 cells stained with ab9474 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.