生物素Anti-Phosphoserine抗体
参阅全部 Phosphoserine 一抗
生物素兔多克隆抗体to Phosphoserine
Rabbit
Biotin
Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
适用于: WB, IP, ELISAmore details
与反应: Species independent
Chemical/ Small Molecule corresponding to Phosphoserine conjugated to keyhole limpet haemocyanin. BSA and KLH-phosphoserine conjugates.
Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 6.00
Preservative: 0.02% Sodium azide
浓度
50 µg 浓度为 0.25 mg/ml
Immunogen affinity purified
Immunoaffinity chromatography with phosphoserine-agarose.
多克隆
IgG
Abpromise™承诺保证使用ab9335于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 4 µg/ml. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. | |
| IP | Use at an assay dependent dilution. | |
| ELISA | Use a concentration of 0.5 µg/ml. |
phospho-Ser antibody
pS antibody
pSER antibody
Western blot - Biotin Anti-Phosphoserine antibody (ab9335)
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.
