HRP Anti-Phosphoserine抗体
参阅全部 Phosphoserine 一抗
HRP兔多克隆抗体to Phosphoserine
Rabbit
HRP
Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
适用于: WB, ELISAmore details
与反应: Species independent
Chemical/ Small Molecule corresponding to Phosphoserine conjugated to keyhole limpet haemocyanin. BSA and KLH-phosphoserine conjugates.
Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
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Liquid
Shipped at 4°C. Store at +4°C.
pH: 6.00
Constituent: Tris buffered saline
浓度
批次浓度范围 100 µg 浓度为 0.25 - 1 mg/ml
Immunogen affinity purified
Immunoaffinity chromatography with phosphoserine-agarose.
多克隆
IgG
Abpromise™承诺保证使用ab9334于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding. | |
| ELISA | Use a concentration of 1 µg/ml. |
phospho-Ser antibody
pS antibody
pSER antibody
Western blot - HRP Anti-Phosphoserine antibody (ab9334)
Western blotting of mouse brain extract with ab9334 at a 1/200 dilution on a 12% SDS gel. 50 µg protein loaded in each lane. Antibody incubated for 2 hours incubation at room temperature.
