Anti-Phosphothreonine抗体
参阅全部 Phosphothreonine 一抗
兔多克隆抗体to Phosphothreonine
Rabbit
Recognize proteins phosphorylated on threonine residues. Not cross-reacted with phosphotyrosine.
适用于: WB, IP, ELISA, IHC-Frmore details
与反应: Mouse, Species independent
Chemical/ Small Molecule corresponding to Phosphothreonine conjugated to keyhole limpet haemocyanin.
Use mouse spleen lysate treated with sodium vanadate or mouse brain lysate for WB. Synthetic phosphopeptide (phosphorylated on threonine) for ELISA.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 6.00
Constituents: PBS, 50% Glycerol
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab9337于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | Use a concentration of 2 µg/ml. |
| IP | Use a concentration of 10 µg/ml. Use at a concentration of 10 µg/mg. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation. | |
| ELISA | Use a concentration of 0.5 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. |
Western blot - Anti-Phosphothreonine antibody (ab9337)
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
ELISA - Anti-Phosphothreonine antibody (ab9337)
Antibody Capture ELISA
Label: immobilized antigen
