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Anti-CD6/T12 antibody [MEM-98]

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  • 产品名称

    Anti-CD6/T12抗体[MEM-98]
    参阅全部 CD6/T12 一抗

  • 描述

    小鼠单克隆抗体[MEM-98] to CD6/T12

  • 宿主

    Mouse

  • 特异性

    CD6 / T12 antigen (human)

  • 经测试应用

    适用于: WBFlow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length native protein (purified) corresponding to Human CD6/T12.

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS

  • 浓度

    • 100 µg 浓度为 1 mg/ml

  • 纯度

    Protein A purified

  • 纯化说明

    Purified from TCS. Purity >95% by SDS-PAGE.

  • 克隆

    单克隆

  • 克隆编号

    MEM-98

  • 骨髓瘤

    unknown

  • 同种型

    IgG1

  • 轻链类型

    unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9181于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
WB

Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 72 kDa. 

works in WB application under non-reducing conditions

Flow Cyt

Use a concentration of 2 µg/ml.

  • 数据库链接

  • 别名

    • CD6 molecule antibody

    • CD6_HUMAN antibody

    • FLJ44171 antibody

    • OX52 antibody

    • T cell differentiation antigen CD6 antibody

    • T-cell differentiation antigen CD6 antibody

    • T12 antibody

    • TP120 antibody

    • CD_antigen=CD6 antibody

    • CD6 antibody

    • CD6 antigen Tp120 antibody

  • Western blot - Anti-CD6/T12 antibody [MEM-98] (ab9181)

    Western blot - Anti-CD6/T12 antibody [MEM-98] (ab9181)

    All lanes : Anti-CD6/T12 antibody [MEM-98] (ab9181) at 1 µg/ml

    Lane 1 : Molt-4 whole cell extracts, reducing
    Lane 2 : HeLa whole cell extracts, reducing
    Lane 3 : Molt-4 whole cell extracts, non-reducing
    Lane 4 : HeLa whole cell extracts, non-reducing

    Secondary
    All lanes : IRDye 800CW Goat-anti-Mouse IgG

    Predicted band size: 72 kDa



    Nitrocellulose membrane blot was probed with ab9181 used at 1 µg/ml, followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.

    CD6 molecules were detected at ~100 kDa in Molt-4 cell line under non-reducing conditions.

  • Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)

    Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)

    Flow cytometric analysis of human CD6 positive lymphocytes (red-filled) / neutrophil granulocytes (black-dashed) from peripheral whole blood cells labeling CD6/T12 with ab9181 at 2µg/ml. Secondary used is a goat anti-mouse-APC.

  • Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)

    Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)

    Flow cytometric analysis of human peripheral whole blood labeling CD6/T12 with ab9181 at 2µg/ml. Secondary used is a goat anti-mouse-APC.