Anti-CD6/T12抗体[MEM-98]
参阅全部 CD6/T12 一抗
小鼠单克隆抗体[MEM-98] to CD6/T12
Mouse
CD6 / T12 antigen (human)
适用于: WB, Flow Cytmore details
与反应: Human
Full length native protein (purified) corresponding to Human CD6/T12.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
Purified from TCS. Purity >95% by SDS-PAGE.
单克隆
MEM-98
unknown
IgG1
unknown
Abpromise™承诺保证使用ab9181于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 72 kDa. works in WB application under non-reducing conditions | |
Flow Cyt | Use a concentration of 2 µg/ml. |
Entrez Gene: 923 Human
Omim: 186720 Human
SwissProt: P30203 Human
CD6 molecule antibody
CD6_HUMAN antibody
FLJ44171 antibody
OX52 antibody
T cell differentiation antigen CD6 antibody
T-cell differentiation antigen CD6 antibody
T12 antibody
TP120 antibody
CD_antigen=CD6 antibody
CD6 antibody
CD6 antigen Tp120 antibody
Western blot - Anti-CD6/T12 antibody [MEM-98] (ab9181)
All lanes : Anti-CD6/T12 antibody [MEM-98] (ab9181) at 1 µg/ml
Lane 1 : Molt-4 whole cell extracts, reducing
Lane 2 : HeLa whole cell extracts, reducing
Lane 3 : Molt-4 whole cell extracts, non-reducing
Lane 4 : HeLa whole cell extracts, non-reducing
Secondary
All lanes : IRDye 800CW Goat-anti-Mouse IgG
Predicted band size: 72 kDa
Nitrocellulose membrane blot was probed with ab9181 used at 1 µg/ml, followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.
CD6 molecules were detected at ~100 kDa in Molt-4 cell line under non-reducing conditions.
Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)
Flow cytometric analysis of human CD6 positive lymphocytes (red-filled) / neutrophil granulocytes (black-dashed) from peripheral whole blood cells labeling CD6/T12 with ab9181 at 2µg/ml. Secondary used is a goat anti-mouse-APC.
Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)
Flow cytometric analysis of human peripheral whole blood labeling CD6/T12 with ab9181 at 2µg/ml. Secondary used is a goat anti-mouse-APC.