Anti-TLR2 antibody [TL2.1]

• 产品名称

  Anti-TLR2抗体[TL2.1]
  参阅全部 TLR2 一抗

• 描述

  小鼠单克隆抗体[TL2.1] to TLR2

• 宿主

  Mouse

• 经测试应用

  适用于: IP, Functional Studies, Flow Cyt, WB, Neutralising, ICC/IFmore details

• 种属反应性

  与反应: Human, Common marmoset
  预测可用于: Dog, Monkey, Cynomolgus monkey, Rhesus monkey

• 免疫原

  Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.

• 阳性对照

• WB: THP1 + LPS. IHC-P: Human TLR2. ICC: Human U937 cells. Flow: Human peripheral blood mononuclear cells.

• 常规说明

  Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Constituents: PBS, 1% BSA

• 浓度

• 批次浓度范围 50 µg 浓度为 0.1 - 100 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  0.2 µm filtered

• Primary antibody说明

  Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.

• 克隆

  单克隆

• 克隆编号

  TL2.1

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab9100于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 519033 Human

• Entrez Gene: 448807 Dog

• Entrez Gene: 7097 Human

• Entrez Gene: 574290 Rhesus monkey

• Omim: 603028 Human

• SwissProt: Q95M53 Cynomolgus monkey

• SwissProt: Q689D1 Dog

• SwissProt: O60603 Human

• SwissProt: B3Y618 Rhesus monkey

别名

• CD282 antibody

• CD282 antigen antibody

• TIL 4 antibody

• TIL4 antibody

• TLR 2 antibody

• TLR2 antibody

• TLR2_HUMAN antibody

• Toll like receptor 2 antibody

• Toll like receptor 2 precursor antibody

• Toll-like receptor 2 antibody

• Toll/interleukin 1 receptor like 4 antibody

• Toll/interleukin 1 receptor like protein 4 antibody

• Toll/interleukin receptor like protein 4 antibody

• Toll/interleukin-1 receptor-like protein 4 antibody

• 

  Flow Cytometry - Anti-TLR2 antibody [TL2.1] (ab9100)

  TLR2 staining on human peripheral blood mononuclear cells by using ab9100. Green: CD3+-cells; Red: CD14+ cells

• 

  Western blot - Anti-TLR2 antibody [TL2.1] (ab9100)

  All lanes : Anti-TLR2 antibody [TL2.1] (ab9100) at 1 µg/ml
  
  Lane 1 : THP1
  Lane 2 : THP1 + LPS
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
  
  Performed under reducing conditions.
  
  
  

  This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was ran at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked with 2% BSA for 60 minutes before being incubated with ab9100 (anti-TLR2 antibody; 1 ug/mL) for 18 hours at 4°C. Antibody binding was detected using ab97040 (HRP-labelled goat anti-mouse IgG)  at  1:50,000 dilutions for 1 hour at room temperature.Visualisation was conducted using ECL development solution ab133406.

  This gel was run under reducing conditions resulting in weak signal. We recommend customers to use non-reducing conditions.