Anti-Bovine Serum Albumin抗体[5F9]
参阅全部 Bovine Serum Albumin 一抗
小鼠单克隆抗体[5F9] to Bovine Serum Albumin
Mouse
The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.
适用于: WB, ELISAmore details
与反应: Cow
不与反应: Chicken, Human
Full length native protein (purified) corresponding to Bovine Bovine Serum Albumin.
Database link: P02769
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
Bovine Serum Albumin monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography and reacts with bovine serum albumin.
单克隆
5F9
Sp2/0
IgG1
unknown
Abpromise™承诺保证使用ab9092于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/2000 - 1/10000. | |
ELISA | 1/120000. |
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Western blot - Anti-Bovine Serum Albumin antibody [5F9] (ab9092)
Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.
Detection occurs using a 1:5000 dilution of IRDye800TM Conjugated Goat-a-Mouse IgG reacted for 1 h at room temperature. Visualization is by near-infrared (IR) western blotting analysis using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR Biosciences, Lincoln, NE.
Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.
Detection occurs using a 1:5000 dilution of IRDye800