Anti-Rhodamine antibody [11H10]

• 产品名称

  Anti-Rhodamine抗体[11H10]

• 描述

  小鼠单克隆抗体[11H10] to Rhodamine

• 宿主

  Mouse

• 特异性

  Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red.

• 经测试应用

  适用于: Dot blot, ELISAmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Chemical/ Small Molecule corresponding to Rhodamine conjugated to keyhole limpet haemocyanin.

• 常规说明

  The molecular formula of 5-carboxytetramethylrhodamine, succinimidyl ester (5-TAMRA, SE)is C₂₉H₂₅N₃O₇ The molecular weight of 5-TAMRA, SE is 527.53 This product contains 0.01% (w/v) Sodium Azide preservative.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 纯度

  Protein A purified

• 纯化说明

  RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives.

• 克隆

  单克隆

• 克隆编号

  11H10

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab9093于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

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  Anti-Rhodamine antibody [11H10] (ab9093)

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  Anti-Rhodamine antibody [11H10] (ab9093)

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  Anti-Rhodamine antibody [11H10] (ab9093)

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  Anti-Rhodamine antibody [11H10] (ab9093)

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  Dot Blot - Anti-Rhodamine antibody [11H10] (ab9093)This image is courtesy of an anonymous abreview.

  A dilution series of two TAMRA labeled peptides were bound to a PVDF membrane using a dot blot apparatus. Peptide #1 is conjugated directly to TAMRA via an amide bond whereas peptide #2 contains an aminohexanoyl linker between the TAMRA and the peptide. Also note that the peptides differ in sequence and in length, peptide #1 has 15 amino acids and peptide #2 has 29 amino acids. A dilution series for each peptide was made starting with approximately 0.5 nmoles of peptide and followed by a series of 1:2 dilutions. After binding of the peptides to the PVDF membrane, the membrane was then blocked in 5% milk in 1X TBST for 1 hour at room temperature. The membrane was then incubated in with the ab9093 antibody at a 1:2000 dilution in 5% milk in 1X TBST for 1 hour at room temperature. Following incubation with the primary antibody the blot was then washed 3 times in 1X TBST and then incubated with a 1:10,000 dilution of an anti-mouse-HRP secondary antibody for 45 minutes at room temperature