Anti-Histone H3 (mono methyl K36)抗体- ChIP Grade
参阅全部 Histone H3 一抗
兔多克隆抗体to Histone H3 (mono methyl K36) - ChIP Grade
Rabbit
Specific for human Histone H3 mono methyl K36. Shows partial cross-reactivity with di-methyl K36 (please see Western Blot image). This antibody may not be suitable for experiments on yeast lysate. Although the antibody is specifically blocked using the immunising peptide, customer feedback indicates that it detects a band using S. cerevisiae K36 point mutants. We welcome further customer feedback.
适用于: WB, ChIP, ICC/IFmore details
与反应: Human
预测可用于: Mouse, Cow, Pig, Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster, Mammals
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ICC/IF: HeLa cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.8 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab9048于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) | 1/1000. |
| ChIP | (3) | Use 2 µg for 25 µg of chromatin. |
| ICC/IF | (1) | Use a concentration of 0.1 µg/ml. |
Entrez Gene: 8350 Human
Entrez Gene: 8351 Human
Entrez Gene: 8352 Human
Entrez Gene: 8353 Human
Entrez Gene: 8354 Human
Entrez Gene: 8355 Human
Entrez Gene: 8356 Human
Entrez Gene: 8357 Human
Entrez Gene: 8358 Human
Entrez Gene: 8968 Human
Entrez Gene: 319152 Mouse
Entrez Gene: 319153 Mouse
Entrez Gene: 360198 Mouse
Entrez Gene: 97908 Mouse
Omim: 602810 Human
SwissProt: P68431 Human
SwissProt: P68433 Mouse
Unigene: 132854 Human
Unigene: 247813 Human
Unigene: 247814 Human
Unigene: 248176 Human
Unigene: 443021 Human
Unigene: 484990 Human
Unigene: 532144 Human
Unigene: 533292 Human
Unigene: 546315 Human
Unigene: 586261 Human
Unigene: 591778 Human
Unigene: 221301 Mouse
Unigene: 261657 Mouse
Unigene: 377874 Mouse
Unigene: 390558 Mouse
Unigene: 397328 Mouse
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ChIP - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab9048 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048)
ab9048 staining Histone H3 (mono methyl K36) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab9048 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048)
All lanes : Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048) at 1/500 dilution
Lane 1 : Histone prep
Lane 2 : Histone prep with Human Histone H3 (mono methyl K36) peptide (ab1783) at 1 µg/ml
Lane 3 : Histone prep with Human Histone H3 (di methyl K36) peptide (ab1784) at 1 µg/ml
Lane 4 : Histone prep with Human Histone H3 (tri methyl K36) peptide (ab1785) at 1 µg/ml
Lane 5 : Histone prep with Human Histone H3 (unmodified ) peptide (ab2623) at 1 µg/ml
Lane 6 : Histone prep with Human Histone H3 (mono methyl K4) peptide (ab1340) at 1 µg/ml
Lysates/proteins at 0.5 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Secondary ab: Alexa Fluor 680 Goat anti-rabbit IgG
0.5µg histone prep used per lane
Primary antibody:
Lane 1: ab9048 (Histone H3 Mono Methyl K36) 1/500
Lane 2: ab9048 (Histone H3 Mono Methyl K36) 1/500 + ab1783 (ab9048) (Histone H3 Mono Methyl K36) peptide 1µg/ml
Lane 3: ab9048 (Histone H3 Mono Methyl K36) 1/500 + ab1794 (ab9049) (Histone H3 Di Methyl K36) peptide 1µg/ml
Lane 4: ab9048 (Histone H3 Mono Methyl K36) 1/500 + ab1785 (ab9050) (Histone H3 Tri Methyl K36) peptide 1µg/ml
Lane 5: ab9048 (Histone H3 Mono Methyl K36) 1/500 + ab2623 (Histone H3 (23-34) – unmodified) peptide 1µg/ml
Lane 6: ab9048 (Histone H3 Mono Methyl K36) 1/500 + ab1340 (ab8895) (Histone H3 Mono methyl K4) peptide 1µg/ml
ab9048 specifically recognise
Western blot - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048)Image courtesy of an anonymous Abreview.
All lanes : Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048) at 1/1400 dilution
All lanes : Whole cell lysate prepared from Drosophila BG3 cells
Lysates/proteins at 500000 cells per lane.
Secondary
All lanes : HRP donkey anti-rabbit monoclonal at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 17,42 kDawhy is the actual band size different from the predicted?
Exposure time: 30 seconds
