Anti-Smoothelin antibody [R4A]

• 产品名称

  Anti-Smoothelin抗体[R4A]
  参阅全部 Smoothelin 一抗

• 描述

  小鼠单克隆抗体[R4A] to Smoothelin

• 宿主

  Mouse

• 特异性

  Reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells.

• 经测试应用

  适用于: ICC/IF, IHC-Fr, IHC-P, WBmore details

• 种属反应性

  与反应: Chicken, Cat, Dog, Human, Pig, Monkey
  不与反应: Mouse, Rat, Hamster

• 免疫原

  Recombinant full length protein corresponding to Chicken Smoothelin. Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal extract of chicken gizzard.
  Database link: A0A8V0ZU10

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  R4A

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8969于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 373982 Chicken

• Entrez Gene: 6525 Human

• Omim: 602127 Human

• SwissProt: P53814 Human

• Unigene: 149098 Human

• 别名

• FLJ38597 antibody

• Smoothelin antibody

• SMSMO antibody

• Smtn antibody

• SMTN_HUMAN antibody

• FLJ35168 antibody

• FLJ35365 antibody

• FLJ35620 antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

  Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).

• 

  Western blot - Anti-Smoothelin antibody [R4A] (ab8969)

  All lanes : Anti-Smoothelin antibody [R4A] (ab8969)
  
  Lane 1 : Human uterine artery
  Lane 2 : Human uterine corpus.
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [R4A] (ab8969)

  Immunohistochemical analysis on paraffin section of human smooth muscle labeling Smoothelin with ab8969.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)

  Indirect immunofluorescence staining of a cultured human fibroblast, showing colocalization of smoothelin (red fluorescence; as detected by ab8969) and actin (green fluorescence).

• 

  Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

  Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).

• 

  Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

  Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)

  Human blood vessel in cardiac muscle stained with the monoclonal smoothelin antibody ab8969.

• 

  Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

  Immunohistochemical analysis on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)This image is courtesy of an anonymous Abreview

  ab8969 staining cells from a human vascular smooth muscle cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. An Rhodamine conjugated donkey anti-mouse antibody, diluted 1/200, was used as the secondary.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

  Immunohistochemistry on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.